Extractive product from the hypophysis gland.



- nrr FRANZ FLAECHER AND BAPTIST REUTER, or H6cHsT-oN-THE-MArN,' GERMANY, ASSIGNORS To FARIBWERKE VORM. MEISTER, LUCIUS & BRi'iNING, 0F HocHsT-' ON-THE-MAIN, GERMANY, A CORPORATION OF GERMANY.

, EXTRACTIVE PRODUCT FROM THE HYPOPHYSIS GLAND.

No Drawing.

To all whom it may concern:

Be it known that we, FRANZ FLAEGHER, Ph. D., chemist, and BAPTIST REUTER, Ph. 1)., chemist, citizens of the Empire of Germany, residing at Hochst-on-the-Main, Germany, have invented certain new and useful Improvements in an Extractive Product from the Hypophysis Gland, of which the following is a specification.

Our invention relates to a new and useful product which is in a stable, permanent and concentrated form the physiologically-eifectire element of the infundibular portion of the hypophysis cerebri, the said product being practically free from inert and deteriorating matter.

It is known that the extract of the said hypophysis possesses a hemostatic, astringent, blood-pressure raisingand labor-pains promoting efiect. Attempts have been made to obtain the efiicient constituent of the gland in a more or less segregated condition. But these attempts have failed to produce a stable, pure and reliable product, uniform and permanent in its action.

Our invention renders available for use the above-mentioned properties of the hypophysis in a stable, pure and concentrated form, without danger of bringing injurious bodies into the patients system.

In order to obtain the product we proceed as follows:

We take the hypophysis extract which is prepared by extracting the well-ground infundibular portion of the hypophysis by means of slightly acidulated water,.completely freeing the filtrate from albumen and evaporating it. The concentrated extract thus obtained is acidified with slightly diluted sulfuric acid, whereupon an aqueous solution of phosphotungstic acid is added until this precipitating agent no longer causes precipitation. The precipitate, which consists of the phosphotungstic compound of the active base, is well washed with water, acidified with sulfuric acid, and mixed and stirred with water so as to form a thin magma, and finally barium hydrate is added until there is a distinct alkaline reaction.

Specification. of Letters Patent.

Patented Mar. 4t, rare.

Application filed September 25, 1912. Serial No. 722,245.

The so-formed phosphotungstate of barium to is filtered off, the filtrate is freed from the dissolved barium by addition of diluted sulfuric acid, taking care to avoid an excess of this acid, and is then evaporated in cacao to crystallization; or the base may be precipi- 5&5 tated by means of alcohol, acetone, etc., washed with alcohol and dried in cacao.

The base thus obtained readily dissolves in water with alkaline reaction, and is difiicultly soluble in alcohol, ether, ethylacetate and acetone, soluble in valkalis and acids. 4 Our new pure and dry product forms a crystalline powder of a faint yellowishwhite color, it is perfectly stable in the air,

and may therefore be stored without deteriorating.

With acids, the substance forms crystalline salts, which possess the same physiological properties as the base itself and constitute new substances not claimed herein but claimed in another application.

Among the chemical characteristics of the substance the following may be enumerated: It is precipitated by the usual alkaloid reagents, such as picric acid, tannic acid, phosphotungstic acid, mercuric chlorid, mercury potassium iodid, a solution of potamium periodid, platinum chlorid, potassium bismuth iodid, etc., etc. When treated with diazotized sulfanilic acid in a solution alkaline with soda, the substance gives a red coloration. It gives the biuret-reaction, and does not yield, on addition of chlorid of iron,

the green coloration characteristic of adrenation, diificultly soluble in alcohol, ether, acetone, ethyl acetate and benzene, readily soluble in acids and alkalis, forming salts with acids, precipitated by the usual alkaloid re- 5 agents, giving a red coloration when treated with diazotized sulfanilic acid in a solution alkaline with sodium carbonate, giving the yellowish-white color when in a solid-com dition.

In testimony whereof, we aflix our signatures in presence of two witnesses.

Witnesses:

JEAN GRUND, CARL GRUND.- 

